6/14/2023 0 Comments Pet30a vector map pdf![]() AglaHsp82.09 and AglaHsp89.76 showed high expressions with treatment at 0 ☌. Western blot analysis showed that AglaHsps were expressed at a range of temperatures, from −10 ☌ to 25 ☌. ![]() coli, where all four proteins were expressed in inclusion bodies. Protein expression vectors (pET30a- AglaHsps) were constructed and used to express AglaHsps in E. Phylogenetic analysis revealed that Hsps from different subfamilies were evolutionarily conserved, and that AglaHsps were highly similar to those of Coleoptera species. Sequence analysis showed that all four Hsps had specific conserved domains. glabripennis Hsp genes, AglaHsp20.43, AglaHsp71.18, AglaHsp82.09, and AglaHsp89.76, were cloned. However, the temperature adaptation mechanisms of A. When insects are subjected to temperature stress, heat shock proteins (Hsps) limit cell damage and improve cell tolerance via their protein folding, localization, and degradation activities. ![]() glabripennis larvae has been facilitated by its high adaptability to low temperature. It is widely distributed in many countries in Asia, Europe, and North America. Anoplophora glabripennis ( Agla) is an important global quarantine pest due to its highly destructive impacts on forests.
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